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GCSE · Last updated November 2026

GCSE Biology (9-1) Exam: free practice test

GCSE Biology is the separate-science biology GCSE, usually taken alongside GCSE Chemistry and GCSE Physics. With AQA (8461) it is assessed by two 1 hour 45 minute papers of 100 marks each: Paper 1 covers cells, organisation, infection and bioenergetics, and Paper 2 covers homeostasis, inheritance and ecology. Exams are sat in the May/June series only.

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About the GCSE Biology exam

GCSE Biology is the separate-science biology GCSE, usually taken alongside GCSE Chemistry and GCSE Physics. With AQA (8461) it is assessed by two 1 hour 45 minute papers of 100 marks each: Paper 1 covers cells, organisation, infection and bioenergetics, and Paper 2 covers homeostasis, inheritance and ecology. Exams are sat in the May/June series only.

What's on the exam

GCSE Biology
Cell Biology
8 key facts
Organisation
8 key facts
Infection and Response
8 key facts
Bioenergetics
8 key facts
Homeostasis and Response
8 key facts
Inheritance, Variation and Evolution
8 key facts
Ecology
8 key facts

Each branch is a topic in the official outline. Members see every key fact on the full mind map. Read the free study guide →

How to pass the GCSE Biology exam

  • 100 marks in 105 minutes is about 1 minute per mark. A 6-mark extended answer deserves about 6 minutes: plan three linked points with a reason for each.
  • Required practicals are examined in the written papers. Know the method, the variables and the safety point for all 10 AQA biology practicals, especially osmosis, enzymes, photosynthesis and the food tests.
  • Command words matter: 'describe' means say what happens, 'explain' means say why, and 'evaluate' needs a judgement backed by evidence from the question.
  • In calculations show every step and give units. Magnification, percentage change in mass, rate (1 ÷ time) and quadrat estimates come up in almost every series.
  • Never say enzymes are 'killed' or that bacteria 'become immune'. Use 'denatured' and 'resistant'; vague words lose marks even when the idea is right.

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Create a free account after the test: your score by topic, a mistakes bank, the complete study notes for the first topic, flashcards and the GCSE Maths Formula Sheet.

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What's in the premium GCSE Biology prep

Premium GCSE Biology prep is studied one topic at a time. Each step starts with that topic's key notes and flashcard deck, then its own question sets of 25, so you master one topic before you move to the next. When every topic is strong, the 6 full mock exams show you're ready.

3,000practice questions, every answer explained
6full-length mock exams, 50 questions each, timed
800flashcards with spaced repetition, sorted by topic
60comprehensive key notes across 7 topics

Your step-by-step study path

  1. 1
    Cell Biology9 key notes, 115 flashcards, then 450 practice questions in 18 sets of 25
    Notes free with an account
  2. 2
    Organisation9 key notes, 115 flashcards, then 425 practice questions in 17 sets of 25
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  3. 3
    Infection and Response9 key notes, 115 flashcards, then 425 practice questions in 17 sets of 25
    Premium
  4. 4
    Bioenergetics9 key notes, 115 flashcards, then 425 practice questions in 17 sets of 25
    Premium
  5. 5
    Homeostasis and Response8 key notes, 115 flashcards, then 425 practice questions in 17 sets of 25
    Premium
  6. 6
    Inheritance, Variation and Evolution8 key notes, 115 flashcards, then 425 practice questions in 17 sets of 25
    Premium
  7. 7
    Ecology8 key notes, 110 flashcards, then 425 practice questions in 17 sets of 25
    Premium
  8. Final step: 6 full mock exams50 questions each, timed like exam day. Hit your target on them before your first paper.
    Premium
Free with an account

Read the complete GCSE Biology study notes for “Cell Biology”

Our first full set of comprehensive notes, about 18 minutes of reading: Animal, plant and bacterial cells · Size, units and magnification · Specialised cells and differentiation · Chromosomes, the cell cycle and mitosis · Stem cells · Culturing microorganisms (separate biology) · Diffusion, osmosis and active transport, a worked exam example, the traps examiners use and a one-minute recap. Create a free account to read it and see how premium notes work before you decide.

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Study tools included

3,000 practice questions

3,000 exam-style questions per exam, organised topic by topic in sets of 25. Every answer explained, with the rule it comes from.

6 full mock exams

6 full-length timed mock exams per exam, with real question counts and time limits, so test day feels like practice day.

Exam Readiness Score

One 0 to 100 number that tells you when you're ready for exam day. Reach 80 and you're covered by our Pass Guarantee.

800 flashcards + spaced repetition

800 flashcards per exam, in a deck for each topic, that come back right before you'd forget them. Five minutes a day keeps every number fresh.

60 comprehensive key notes

60 key notes per exam, split across its topics and each explained in full: the concepts, worked exam examples, the traps examiners use and a quick recap. Your first set is free with an account.

Mind maps

See the whole exam on one screen: every topic and the facts under it.

Sample questions with answers

These are the questions in the free test above, with answers. Try the test first. You'll learn more by getting some wrong.

1. A drawing of a cell is 30 mm long. The real cell is 0.06 mm long. What is the magnification of the drawing?
Answer: × 500. magnification = size of image ÷ size of real object = 30 ÷ 0.06 = 500. Both lengths are already in mm, so no unit conversion is needed.
2. Potato cylinders are placed in a concentrated sugar solution for 30 minutes and lose mass. Which statement explains this?
Answer: Water moves out of the cells by osmosis, from a dilute solution to a more concentrated solution. The sugar solution is more concentrated than the cell contents, so water leaves the cells by osmosis across the partially permeable membrane and the cylinders lose mass.
3. Why does the rate of an enzyme-controlled reaction fall sharply above about 45 °C?
Answer: The shape of the active site changes, so the substrate no longer fits. High temperatures denature the enzyme: the bonds holding its shape break and the active site changes shape. Enzymes are proteins, not living things, so 'killed' scores no marks.
4. Which chamber of the heart pumps oxygenated blood out to the rest of the body?
Answer: Left ventricle. The left ventricle pumps oxygenated blood into the aorta. Its wall is the thickest because it pumps blood all round the body; the right ventricle only pumps blood to the lungs.
5. Why are antibiotics not used to treat measles?
Answer: Measles is caused by a virus, and antibiotics do not kill viruses. Measles is a viral disease. Antibiotics kill bacteria; viruses live and reproduce inside cells, so antibiotics have no effect on them.
6. What does a vaccine contain?
Answer: Small amounts of a dead or inactive form of the pathogen. A vaccine contains dead or inactive pathogen. White blood cells respond by making antibodies, so if the live pathogen enters later the antibodies are made quickly and the person does not become ill.
7. Which is the balanced symbol equation for photosynthesis?
Answer: 6CO₂ + 6H₂O → C₆H₁₂O₆ + 6O₂. Carbon dioxide and water are the reactants; glucose and oxygen are the products. The first option is aerobic respiration and the third is fermentation by yeast.
8. A lamp is moved from 10 cm to 20 cm away from a pondweed plant. What happens to the light intensity reaching the plant?
Answer: It falls to one quarter. Light intensity ∝ 1 ÷ distance². Doubling the distance gives 1 ÷ 2² = 1/4 of the original intensity.
9. During vigorous exercise, what is produced by anaerobic respiration in human muscle cells?
Answer: Lactic acid. In muscles, anaerobic respiration is glucose → lactic acid. Ethanol and carbon dioxide are made by anaerobic respiration in yeast and plant cells.
10. Which hormone does the pancreas release when the blood glucose concentration is too high?
Answer: Insulin. Insulin causes glucose to move from the blood into cells, and the liver and muscles store it as glycogen. Glucagon is released when blood glucose is too low.
11. What is the correct order of structures in a reflex arc?
Answer: Receptor → sensory neurone → relay neurone → motor neurone → effector. The receptor detects the stimulus, the sensory neurone carries the impulse to the relay neurone in the spinal cord, and the motor neurone carries it to the effector (a muscle or gland).
12. Two parents are both carriers (Ff) of cystic fibrosis, which is caused by a recessive allele. What is the probability that their child will have cystic fibrosis?
Answer: 25%. Ff × Ff gives FF, Ff, Ff and ff. Only ff (1 out of 4) has the disorder, so the probability is 25%. The chance of a carrier (Ff) is 2 out of 4, or 50%.
13. How many chromosomes are in a human sperm cell?
Answer: 23. Gametes are made by meiosis and contain a single set of chromosomes: 23. Fertilisation restores the full number of 46 (23 pairs).
14. A student places 10 quadrats, each 0.25 m², in a field of 200 m² and counts 30 daisies in total. What is the estimated daisy population of the field?
Answer: 2400. Area sampled = 10 × 0.25 = 2.5 m². Daisies per m² = 30 ÷ 2.5 = 12. Population = 12 × 200 = 2400.
15. Why is only about 10% of the biomass at one trophic level transferred to the next level?
Answer: Biomass is lost as waste such as faeces and urea, and much of the glucose is used in respiration. Not all of an organism is eaten or absorbed, some is egested as faeces or excreted as urea, and a lot of glucose is respired to release energy, producing carbon dioxide and water rather than new biomass.

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Exam guide

GCSE Biology exam guide (2027): GCSE Biology (9-1) is the separate-science biology GCSE, often called triple science biology. Whichever board your school uses, you sit two written papers of 1 hour 45 minutes at the end of Year 11. This guide uses AQA 8461, the most widely taken specification, and notes where Pearson Edexcel (1BI0) and OCR Gateway (J247) differ.

GCSE Biology exam FAQ

How many questions are on the GCSE Biology exam?

AQA: 2 written papers, 100 marks each (200 marks in total). Edexcel 1BI0 is also 2 × 100 marks; OCR Gateway J247 is 2 × 90 marks, with a time limit of 3 hours 30 minutes in total (2 × 1 hour 45 minutes); the same for aqa, edexcel and ocr. Format: written exam, calculator allowed on both papers. foundation tier (grades 1 to 5) or higher tier (grades 4 to 9), and both papers must be taken at the same tier.

What is the passing score for GCSE Biology?

Grade 4 is a standard pass and grade 5 a strong pass. Boundaries are set after every exam series, so aim for 70% in practice.

How much does the GCSE Biology exam cost?

No fee for school and college students (the school pays). Private candidates pay the exam centre; fees vary by centre. School and college students don't pay exam fees themselves. Private candidates pay the exam centre, and fees vary by centre and board.

Which languages is the GCSE Biology exam offered in?

English.

Where do I take the GCSE Biology exam?

At your school or college, or as a private candidate at an exam centre that accepts them.

Is this GCSE Biology practice test free?

Yes. The 15-question practice test on this page is free, with an explanation for every answer and no signup. A free account also unlocks the complete study notes for the first topic. The full GCSE Biology prep is Premium at £29 a month for this exam.

What's in the premium GCSE Biology prep?

3,000 practice questions organised topic by topic (Cell Biology: 450; Organisation: 425; Infection and Response: 425; Bioenergetics: 425; Homeostasis and Response: 425; Inheritance, Variation and Evolution: 425; Ecology: 425), 6 full-length timed mock exams of 50 questions, 800 flashcards and 60 comprehensive key notes split across all 7 topics, plus a mistakes bank and the Readiness Score.

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Exam details are summarised from the exam boards' published specifications available in November 2026. the exam boards changes formats and fees, so confirm with AQA, Pearson Edexcel and OCR before you book. Kennington College is not affiliated with any exam board or with Ofqual.

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